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A cytochrome P450 1A (CYP1A) cDNA was isolated from an adult zebrafish (Danio rerio) library. The 2580-bp clone (GenBank Accession No. AF210727) contained a 62-bp 5′-unstranslated region (UTR), 1557-bp coding region and 962-bp 3′-UTR. The deduced 519-residue protein (calculated molecular weight 58,556, pI = 7.58) shared 74% identity with rainbow trout CYP1A and 57 and 54% identities with mouse and...
The hepatic expression of a recently reported cytochrome P450, P450 2K1 (CYP2K1), was studied in livers of rainbow trout (Oncorhynchus mykiss) 30 day embryos (1 day before hatching) and 2 day and 10 day sac-fry at both the transcriptional and translational levels using a CYP2K1 cDNA probe (2K1, 7c, 440 bp) and antibodies (polyclonal and monoclonal) against rainbow trout cytochrome P450 LMC2, the presumed...
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