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To reveal the brewing mechanism of Sichuan Xiaoqu liquor, microbial communities in Xiaoqu, a saccharification and fermentation agent, were characterized using microbial isolation and identification techniques, MiSeq high‐throughput sequencing and bioinformatics. From Xiaoqu commonly used in three different regions (Dazhu, Pengzhou and Hubei) in China, three yeast strains and seven moulds were isolated...
A bench-scale experimental study was carried out to investigate the overall performance of a combined anaerobic reactor (CAR)for treating mustard tuber wastewater at organic loading. The experimental results show that Under the conditions that temperature is 30°C and loading is 7.5 kgCOD/(m3×d), the influent mustard tuber wastewater with salinity of 1.8(Cl-) and COD of 4500mg/L is treated to 500mg/L...
The bio-dopp process was used to treat papermaking midcourse wastewater. The CODcr, BOD5 and color removal rate were investigated through the self-made experimental system. The CODcr removal rate of reactor was higher than 90%, the BOD5 removal rate reached 96~99% as the BOD5 of effluent was less than 10 mg/L, the chroma of effluent reached 50 multiple. Moreover, the sludge concentration was about...
To detect and identify of Enterohemorrhagic Escherichia coli O157:H7, the gene rfbE of O157:H7 was detected as the aim gene and the Agilent 2100 bioanalyzer was used as an analysis tool, which was compared with the conventional agarose gel electrophoresis. Finally, it was known that the rfbE gene was successful in detection of O157:H7. And the bioanalyzer was as 10 times sensitive as the agrouse gel...
To explore the feasibility of using Agilent 2100 Bioanalyzer in the detection of the three kinds of food-borne pathogens, three pairs of primers were designed according to invA gene of Salmonella ipah gene of Shigella and inv gene of Yersinia pseudotuberculosis, and then the single PCR was performed to detect specificity. Those genes were amplified by Multiplex PCR technique using specific primers,...
Biological phosphate reduction was observed in the authors' previous studies in an anaerobic sequencing batch reactor (ASBBR) in treating preserved pickle wastewater with high salinity and phosphorus concentration. Key factors such as temperature, pH value, organic loading and influent nitrate concentration that influence this process in terms of phosphorus removal efficiency are investigated in this...
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