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Effective cryopreservation protocols are essential for long-term storage of cells and their subsequent clinical application. Freezing protocols are generally considered as safe; however, putative effects on epigenetic marks have not yet been studied in detail. While post-thaw cell survival rates have been used to evaluate the success of cryopreservation protocols, increasing evidence suggests that...
Cryopreservation is routinely used to store different types of cells and tissues. It is used for transportation and cell banking. However, the impact of cryopreservation on the genetic and epigenetic profile of frozen cells is still unclear. Here, we try to explore the alterations caused to cryopreserved mesenchymal stem cells and its possible implications. Our freezing protocol showed that the cells...
Cryopreservation plays an important role in the long-term storage of cells and tissues. A high survival rate is a function of optimal cooling rate, appropriate cryoprotective agent (CPA) and its adjusted concentration. The most widely used CPA, dimethyl sulfoxide (Me 2 SO), however is toxic at high concentrations and has detrimental effects on the biological functioning of a cell. Therefore,...
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