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An HPLC-DAD method for determining corticoids in calf feed and in animal feeding water samples using a monolithic column has been developed and validated. The method optimization included the study of binary mobile phases of water and acetonitrile. The optimum separation was achieved at 40 °C, with acetonitrile:H2O 29:71 v/v used as mobile phase and a 3 ml/min flow-rate, which resulted in their separation...
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