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We evaluate the damage on living cells induced by electron beam irradiation. Cell viability is observed with a fluorescence microscope. Electron beam induced damage is evaluated by the comparison of fluorescence images obtained before and after the electron beam irradiation. After electron beam irradiation, a bleb related to cell injury was observed and cell viability signal was decreased on the irradiated...
This study analyze the intensity distribution of CL generated in fluorescent thin film by combination of Monte Carlo method and finite difference time domain (FDTD) method. The Monte Carlo method analyzes the electron beam scattering in the fluorescent thin film and FDTD method analyzes the light propagation of CL. From analysis result, the spot size of CL at surface of 50 nm Y2O3: Eu3+ is 43 nm....
We present superresolving optical imaging system and demonstrate the observation of biological specimens without any stained process. The electron-beam excited assisted (EXA) optical microscope has a few tens nanometer spatial resolution laterally and is possible to observe dynamic behaviors of specimens in various surroundings such as air or liquids. In the EXA-microscope, a nano-light source in...
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