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Semipreparative high‐speed counter‐current chromatography (HSCCC) by time‐controlled collection method was successfully applied for isolation and purification of α‐terthienyl, 5‐(3‐buten‐1‐ynyl)‐2,2′‐bithienyl, and 5‐(3‐penten‐1‐ynyl)‐2,2′‐bithienyl from Flaveria bidentis (L.) Kuntze for the first time. The two‐phase solvent system composed of n‐hexane and acetonitrile at the volume ratio of 1:1 (v/v)...
The first preparative separation of a flavonoid sulphate isorhamnetin 3‐sulphate from Flaveria bidentis (L.) Kuntze by counter‐current chromatography (CCC) was presented. Two kinds of solvent systems were used. A conventional organic/aqueous solvent system n‐butanol–ethyl acetate–water (4:1:5, v/v) was used, yielding isorhamnetin 3‐sulphate 2.0 mg with a purity of 93.4% from 83 mg of pre‐enriched...
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