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Two FMRFamide-related peptides (FaRPs) have been isolated and sequenced from the whole gut of Locusta migratoria L. Peptides were extracted from 500 locust whole guts and separated using reversed-phase high performance liquid chromatography (RP-HPLC). Fractions containing FMRFamide-like immunoreactive (FLI) material were identified using radioimmunoassay (RIA). Sequencing of fractions, using tandem...
SchistoFLRFamide (PDVDHVFLRFamide) is an insect neuropeptide which inhibits spontaneous and induced contractions of locust oviduct. The active core for inhibition lies within the sequence HVFLRFamide, whereas the core for binding lies within the sequence VFLRFamide. This latter peptide shows activity reversal, possessing minor stimulatory activity. The bioassay and receptor binding assay were employed...
In the oviduct of Locusta migratoria, an inhibitory neuropeptide, PDVDHVFLRFamide (SchistoFLRFamide) has separate binding and activation regions. VFLRFamide is the minimum sequence required for binding, which is comparable to the parent peptide, whereas the His residue, which does not contribute to binding, is a critical amino acid for the inhibitory activity of the receptor. In this study, the...
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