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Haemophilus influenzae was labeled with thymidine- 3 H (dThd), then grown in the presence of 5-bromodeoxyuridine (BrdUrd), and then irradiated with 313 nm light (a wavelength that selectively photolyzes DNA containing 5-bromouracil [BrUra]). Irradiation with 313 nm light induced breaks in the 3 H-labeled strands in cells grown with BrdUrd at a much higher frequency than in 14 ...
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