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For construction of xylose-inducible expression vector, 428 bp of xylF promoter was ligated between AatII and HindIII of pUC18, and named pXF428. To investigate the role of XylR protein encoded by xylR on xylF promoter, 1,988 bp of xylR including its promoter was ligated downstream of multiple cloning site (MCS) in the opposite direction of xylF promoter, and named pXFR428. For the measurement of...
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