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Mycobacterium tuberculosis accumulates large amounts of triacylglycerol (TAG) which acts as storage compounds for energy and carbon. The mycobacterial triacylglycerols stored in the form of intracellular lipid droplets are essential for long‐term survival of M. tuberculosis during a dormant state. We report here that when the M. tuberculosis mycolytransferase Ag85A is overexpressed in Mycobacterium smegmatis...
Mycobacterium tuberculosis H37Rv contains the kshA (Rv3526) and kshB (Rv3571) genes, encoding 3‐ketosteroid 9α‐hydroxylase (KSH). Consistent with their predicted roles, the ΔkshA and ΔkshB deletion mutants of M. tuberculosis H37Rv were unable to use cholesterol and 4‐androstene‐3,17‐dione as primary carbon and energy sources. Interestingly, ΔkshA and ΔkshB mutants were also unable to metabolize the...
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