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Bioassays need to be explicit, reproducible and therefore reliable, and have the highest sensitivity possible. It has previously been demonstrated that high surface area silica aggregates can serve as substrates for oligonucleotide synthesis in conventional DNA synthesis machines, and that high sensitivity is observed in subsequent DNA capture and detection in DNA microarray-based assays. However,...
One way to increase the sensitivity of DNA diagnostic assays developed on microarrays is to improve the solid phase that allows the extraction of the target from a biological sample, before detection. Two parameters are influencing the performances of this capture step: (i) the specific surface area being offered for the capture and (ii) the number and the accessibility of oligonucleotide probes immobilized...
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