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A sensitive and specific method for the determination of colchicine in urine, serum, plasma and whole blood has been developed, using HPLC coupled to mass spectrometry with pneumatically assisted electrospray ionization. A simple alcaline (pH = 8.0) extraction of 1 mL of biological fluid was performed with a mixture of diethylether: methylenechloride (7:4, v/v). The organic layer was evaporated to...
An LC/MS method with electrospray-type ionization and selected ion monitoring detection for the determination of buprenorphine (B) and norbuprenorphine (N) in serum and urine is presented, using deuterated B as internal standard.2 mL of serum or urine are subjected to enzymatic hydrolysis (12 h, 37°C), then alkalinized aliquots are applied on Extrelut-3 extraction columns. Elution is performed with...
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