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Reverse transcription quantitative polymerase chain reaction (RT‐qPCR) is widely used in diagnosis and research to determine specific mRNA expressions in cells. As RT‐qPCR applications increase, it's necessary to provide undergraduates hands‐on experience of this modern technique. Here, we report a 3‐week laboratory exercise using RT‐qPCR to demonstrate the light‐dependent expressions of AtRBCS1A...
The GB virus C/hepatitis G virus (GBV-C/HGV) is a Flaviviridae member that despite its nonpathogenicity, has become of great interest given that it could inhibit the replication of the human immunodeficiency virus (HIV). Therefore, a better knowledge of the viral protein E2 has become our aim. In this study, a GBV-C model cell system (HuhEG) which expressing a fusion protein of the GBV-C E2 protein...
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