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Enzymes that act on chitin, including glycoside hydrolases (GH) family 18 (chitinases) and family 20 (NAGases), may have multiple roles in fungal biology. Here, we describe the number, structure and expression of the eleven GH18 and four GH20 genes identified in the wood-rotting basidiomycete Phanerochaete chrysosporium, and measure endochitinase and NAGase activities in situ. A phylogenetic analysis...
Glycoside hydrolase family 18 contains hydrolytic enzymes with chitinase or endo-N-acetyl-β-d-glucosaminidase (ENGase) activity, while glycoside hydrolase family 20 contains enzymes with β-N-acetylhexosaminidase (NAGase) activity. Chitinases and NAGases are involved in chitin degradation. Chitinases are phylogenetically divided into three main groups (A, B and C), each further divided into subgroups...
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