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To investigate the mobile gene pool present in wastewater environments, total community DNA was obtained from two distinct raw effluents: urban and slaughterhouse wastewaters. Bacterial community structure was evaluated by DGGE analysis of 16S rRNA gene fragments. Detection of broad-host-range plasmid sequences and integrase encoding genes was carried out through PCR and Southern hybridization. Gene...
Class 1 integrons are assumed to play an important role in dissemination of antibiotic resistance genes. Class 1 integrons from clinical isolates often have 5′- and 3′-conserved segments flanking the variable region where gene cassettes integrate. Primers targeting the conserved segments were used to amplify the variable region from total community DNA of manure and soil. Integron gene cassette diversity...
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