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Phage Φ29 infects Bacillus subtilis and ejects its linear DNA with a right to left polarity in a two-step, “push–pull” mechanism. In the first step 65% of the DNA is pushed inside the cell, presumably by the pressure built inside the capsid. In the second step, the remaining DNA is pulled by a hypothetical motor that comprises at least viral protein p17, encoded by the right early operon, in an energy-dependent...
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