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A β-galactosidase gene (β-Gal II) from Bifidobacterium adolescentis DSM 20083 was cloned into a pbluescript SK (−) vector and expressed in Escherichia coli. The recombinant enzyme was purified from the cell extract by anion-exchange and size-exclusion chromatography. β-Gal II had a native molecular mass of 235 kDa and the subunits had a molecular mass of 81 kDa, indicating that β-Gal II occurs as...
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