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We have isolated the Saccharomyces cerevisiae pex12 – 1 mutant from a screen to identify mutants defective in peroxisome biogenesis. The pex12Δ deletion strain fails to import peroxisomal matrix proteins through both the PTS1 and PTS2 pathway. The PEX12 gene was cloned by functional complementation of the pex12 – 1 mutant strain and encodes a polypeptide of 399 amino acids. ScPex12p is orthologous...
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