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Previously, we showed that DNA replacement of the seed-containing region of class I siRNAs significantly reduced the off-target effect without substantial loss of target gene silencing activity (Ui-Tei et al, Nucleic Acids Res., 36, 2136-2151, 2008). Separately, we also showed that the level of the off-target effect due to non-modified class I siRNA is determined primarily by the calculated melting...
The capability to induce off-target effects by small interfering RNA (siRNA) is highly correlated with the calculated melting temperature (Tm) of the protein-free seed-target duplex, indicating that its thermodynamic stability is a major factor in determining the degree of the off-target effect. In this study, we analyzed changes in microarray expression profiles following transfection of short hairpin...
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