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The 5' upstream region (-448/-443) of the human dipeptidyl peptidase IV gene promoter containing a consensus E-box (CACGTG) was shown to bind upstream stimulatory factor using nuclear extracts from mouse (3T3) fibroblasts and the human intestinal and hepatic epithelial cell lines Caco-2 and HepG2. Supershift analysis with specific antibodies to USF-1 and USF-2 indicates that USF-1 is the primary isoform...
The distribution of aminopeptidase P was examined in rat small intestinal epithelial cells using the substrate Gly-Pro-Hyp. Soluble activity accounted for 65% of the total activity in these cells while 27% was membrane bound and localized to the brush border membrane fraction. Soluble and brush border membrane associated activities differed with respect to their pH optima, kinetic constants, and...
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