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The paper describes an introductory characterization of antigenic stimulation of excretion–secretion products (ESP) of Gnathostoma binucleatum advanced third-stage larvae cultured in vitro and proteinases present in this products. Excretory and secretory proteins were obtained after 10 larvae were maintained in 5% CO 2 RPMI medium. The supernatant was collected each week for two months. The...
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