Search results for: Atsuo Ito

Items from 1 to 20 out of 65 results

article

Hollow ZnO Nanospheres Enhance Anticancer Immunity by Promoting CD4⁺ and CD8⁺ T Cell Populations In Vivo

Xiupeng Wang, Xia Li, Atsuo Ito, Yu Sogo, more

Small > 13 > 38 > n/a - n/a

Appropriate adjuvant aiding in generating robust anticancer immunity is crucial for cancer immunotherapy. Herein, hollow ZnO (HZnO) nanospheres are synthesized by a facile method using carbon nanospheres as the template. The HZnO nanospheres significantly promote the cellular uptake of a model antigen, and cytokine secretion by antigen‐presenting cells in vitro. HZnO loaded with ovalbumin and polyinosinic‐polycytidylic...

article

Reducing the risk of impaired bone apposition to titanium screws with the use of fibroblast growth factor-2−apatite composite layer coating

Kengo Fujii, Atsuo Ito, Hirotaka Mutsuzaki, Shinji Murai, more

Journal of Orthopaedic Surgery and Research > 2017 > 12 > 1 > 1-9

Background Loosening of screws is a common problem in orthopedic and maxillofacial surgery. Modifying the implant surface to improve the mechanical strength of screws has been tried and reported. We developed screws coated with fibroblast growth factor-2 (FGF-2)−apatite composite layers. We then showed, in a percutaneous external fixation model, that this composite layer had the ability to hold and...

article

Reprint of “Biomimetic apatite coating on yttria-stabilized tetragonal zirconia utilizing femtosecond laser surface processing”

Ayako Oyane, Masayuki Kakehata, Ikuko Sakamaki, Alexander Pyatenko, more

Surface and Coatings Technology > 2016 > 307 > PB > 1144-1151

Yttria-stabilized tetragonal zirconia polycrystals (Y-TZP) have been used in orthopedic and dental implants because of their excellent physicochemical properties. In this study, we developed an apatite coating technique with low thermal effects for Y-TZP ceramics based on a biomimetic process using a supersaturated calcium phosphate solution (CP solution) as a coating medium. To achieve this, we performed...

article

Hollow Structure Improved Anti‐Cancer Immunity of Mesoporous Silica Nanospheres In Vivo

Xiupeng Wang, Xia Li, Atsuo Ito, Kazuko Yoshiyuki, more

Small > 12 > 26 > 3510 - 3515

Hollow and non‐hollow mesoporous silica nanospheres are synthesized and used for cancer vaccine adjuvants. The hollow structure of mesoporous silica nanospheres significantly promote cellular uptake of a model cancer antigen by macrophage‐like cells in vitro, improve anti‐cancer immunity, CD4⁺ and CD8⁺ T cell populations in splenocytes of mice in vivo.

article

Silica Nanospheres: Hollow Structure Improved Anti‐Cancer Immunity of Mesoporous Silica Nanospheres In Vivo (Small 26/2016)

Xiupeng Wang, Xia Li, Atsuo Ito, Kazuko Yoshiyuki, more

Small > 12 > 26 > 3602 - 3602

Hollow and non‐hollow mesoporous silica nanospheres are synthesized and used for cancer vaccine adjuvants by X. Wang, A. Ito, N. M. Tsuji, and co‐workers on page 3510. The hollow structure of mesoporous silica nanospheres significantly promotes cellular uptake of a model cancer antigen by macrophage‐like cells in vitro, improves anti‐cancer immunity, and increases CD4+ and CD8+ T cell populations...

article

Biomimetic apatite coating on yttria-stabilized tetragonal zirconia utilizing femtosecond laser surface processing

Ayako Oyane, Masayuki Kakehata, Ikuko Sakamaki, Alexander Pyatenko, more

Surface and Coatings Technology > 2016 > 296 > C > 88-95

article

Controlled superficial assembly of DNA–amorphous calcium phosphate nanocomposite spheres for surface-mediated gene delivery

Ayako Oyane, Hiroko Araki, Maki Nakamura, Yoshiki Shimizu, more

Colloids and Surfaces B: Biointerfaces > 2016 > 141 > C > 519-527

Surface-mediated gene delivery systems have many potential applications in tissue engineering. We recently fabricated an assembly consisting of DNA–amorphous calcium phosphate (DNA–ACP) nanocomposite spheres on a polymer substrate via coprecipitation in a labile supersaturated calcium phosphate (CaP) solution and demonstrated the assembly’s high gene delivery efficacy. In this study, we conducted...

article

Rod-shaped and substituted hydroxyapatite nanoparticles stimulating type 1 and 2 cytokine secretion

Xiupeng Wang, Xia Li, Atsuo Ito, Yohei Watanabe, more

Colloids and Surfaces B: Biointerfaces > 2016 > 139 > C > 10-16

A Th1 immune response is required for modern vaccines as the most commonly used alum adjuvant has weak capacity for inducing Th1 immune response. Herein, rod-shaped hydroxyapatite (HA), magnesium-substituted HA (MgHA) and zinc-substituted HA (ZnHA) nanoparticles with irregular nanopores were synthesized and used as immunoadjuvants. Magnesium and zinc substitution in HA showed no influence on morphology,...

article

Stimulation of In Vivo Antitumor Immunity with Hollow Mesoporous Silica Nanospheres

Xiupeng Wang, Xia Li, Atsuo Ito, Yohei Watanabe, more

Angewandte Chemie International Edition > 55 > 5 > 1899 - 1903

The use of appropriate adjuvants that support the generation of robust and long‐lasting antitumor immune responses is crucial for tumor immunotherapy owing to the immunosuppressive environment of the growing tumor. However, the most commonly used adjuvant, aluminum hydroxide, is ineffective for generating such immune responses and therefore not suitable for cancer immunotherapy. It is now shown that...

article

Stimulation of In Vivo Antitumor Immunity with Hollow Mesoporous Silica Nanospheres

Xiupeng Wang, Xia Li, Atsuo Ito, Yohei Watanabe, more

Angewandte Chemie > 128 > 5 > 1931 - 1935

article

Effects of gatifloxaine content in gatifloxacine-loaded PLGA and β-tricalcium phosphate composites on efficacy in treating osteomyelitis

Kaori Kimishima, Tomonori Matsuno, Jun Makiishi, Gaku Tamazawa, more

Odontology > 2016 > 104 > 1 > 105-113

Composites of gatifloxacin (GFLX)-loaded poly (lactic-co-glycolic) acid (PLGA) and β-tricalcium phosphate (βTCP) containing 0, 1, and 10 wt % GFLX (0, 1, and 10 wt % GFLX composites), and GFLX-loaded PLGA containing 1, 5, and 10 wt % GFLX (1, 5, and 10wt % GFLX-PLGA) as controls were fabricated and characterized in vitro and in vivo. On in vitro evaluation, the 10 wt % GFLX composite released GFLX...

article

Interlaboratory studies on in vitro test methods for estimating in vivo resorption of calcium phosphate ceramics

Atsuo Ito, Yu Sogo, Atsushi Yamazaki, Mamoru Aizawa, more

Acta Biomaterialia > 2015 > 25 > C > 347-355

A potential standard method for measuring the relative dissolution rate to estimate the resorbability of calcium-phosphate-based ceramics is proposed. Tricalcium phosphate (TCP), magnesium-substituted TCP (MgTCP) and zinc-substituted TCP (ZnTCP) were dissolved in a buffer solution free of calcium and phosphate ions at pH 4.0, 5.5 or 7.3 at nine research centers. Relative values of the initial dissolution...

article

Laser-assisted biomimetic process for surface functionalization of titanium metal

Ayako Oyane, Nao Matsuoka, Kenji Koga, Yoshiki Shimizu, more

Colloid and Interface Science Communications > 2015 > 4 > Complete > 5-9

Biomimetic calcium phosphate (CaP) precipitation processes using supersaturated CaP solutions are useful in surface functionalization of biomedical materials. We applied our laser-assisted biomimetic (LAB) process to successfully achieve rapid single-step CaP precipitation on the surface of titanium metal, which is an important metallic biomaterial, by applying pulsed laser irradiation to the titanium...

article

Improved gene transfer efficiency of a DNA-lipid-apatite composite layer by controlling the layer molecular composition

Yushin Yazaki, Ayako Oyane, Hideo Tsurushima, Hiroko Araki, more

Colloids and Surfaces B: Biointerfaces > 2014 > 122 > C > 465-471

Surface-mediated nonviral gene transfer systems using biocompatible apatite-based composite layers have potential use in tissue engineering applications. Herein, we investigated a relatively efficient system based on a DNA-lipid-apatite composite layer (DLp-Ap layer): an apatite (Ap) layer with immobilized DNA and lipid (Lp) complexes (DLp complexes). DLp-Ap layers were fabricated on substrates using...

article

Pore size‐dependent immunogenic activity of mesoporous silica‐based adjuvants in cancer immunotherapy

Xiupeng Wang, Xia Li, Atsuo Ito, Yu Sogo, more

Journal of Biomedical Materials Research Part B: Applied Biomaterials > 102 > 4 > 967 - 974

Commonly used aluminum hydroxide (Alum) adjuvant provokes a strong type 2 helper T cell (Th2) response for mediating antibody production but is rather ineffective for disease prevention that requires type 1 helper T cell (Th1) response for mediating cellular immunity in human vaccination. Here, for the purpose of inducing Th1 antitumor immunity, a mesoporous silica (MS)‐based adjuvant is prepared...

article

Particle-size-dependent toxicity and immunogenic activity of mesoporous silica-based adjuvants for tumor immunotherapy

Xiupeng Wang, Xia Li, Atsuo Ito, Yu Sogo, more

Acta Biomaterialia > 2013 > 9 > 7 > 7480-7489

Conventionally used adjuvants alone are insufficient for triggering cell-mediated immunity, although they have been successfully developed to elicit protective antibody responses in some vaccines. Here, with the aim of eliciting cell-mediated immunity, pathogen-associated molecular patterns (PAMPs) were immobilized with apatite within the pores and on the surface of mesoporous silica (MS) with particle...

article

Therapeutic effect of zinc‐containing calcium phosphate suspension injection in thermal burn‐rats

Makoto Otsuka, Masayuki Shikamura, Kuniko Otsuka, Yu Sogo, more

Journal of Biomedical Materials Research Part B: Applied Biomaterials > 101A > 5 > 1518 - 1524

The aim of this study was to evaluate the efficacy of suspensions of zinc‐containing tricalcium phosphate (TCP) in the healing of thermal burns in rats. β‐ZnTCP containing 10 mol % zinc, α‐ZnTCP containing 0.9 mol % zinc, and ZnSO₄·(H₂O)₇ (ZnSO₄) were used. The injections were prepared to suspend ZnSO₄, α‐ZnTCP, and β‐ZnTCP powders in 2 mL of 1% sodium alginate saline solution containing 2 mg of Zn...

article

Angiogenesis therapy for brain infarction using a slow-releasing drug delivery system for fibroblast growth factor 2

Yoshiro Ito, Hideo Tsurushima, Masayuki Sato, Atsuo Ito, more

Biochemical and Biophysical Research Communications > 2013 > 432 > 1 > 182-187

Although fibroblast growth factor 2 (FGF2) is a promising agent for treating brain infarction, current methods of FGF2 administration are associated with a short circulating half-life. An FGF2 apatite coating was developed as a slow-releasing drug delivery system (DDS) by forming an FGF2/calcium phosphate composite layer. Hydroxyapatite was coated with high or low doses of FGF2, denoted as FGF-high...

article

Correlation between cell attachment areas after 2h of culture and osteogenic differentiation activity of rat mesenchymal stem cells on hydroxyapatite substrates with various surface properties

Kan Cheng, Motohiro Hirose, Xiupeng Wang, Yu Sogo, more

Biochemical and Biophysical Research Communications > 2013 > 430 > 1 > 156-160

The initial attachment of mesenchymal stem cells (MSCs) to substrates and osteogenic differentiation are supported by culture on a hydroxyapatite substrate. Cell attachment areas of rat MSCs after 2h of culture on hydroxyapatite substrates with various microstructures and the osteogenic differentiation activity thereafter were measured. The perceived outcome was that, after 2h of culture, rat MSCs...

article

DNA-lipid-apatite composite layers enhance gene expression of mesenchymal stem cells

Xiupeng Wang, Atsuo Ito, Xia Li, Yu Sogo, more

Materials Science & Engineering C > 2013 > 33 > 1 > 512-518

DNA-apatite composite layer (D-Ap layer) and DNA-lipid-apatite composite layer (DLp-Ap layer) were prepared on ceramic hydroxyapatite disk and scaffold using supersaturated calcium phosphate solutions supplemented with 0.5–5μg/mL plasmid and 0–10μL/mL lipid transfection reagent FuGENE®. Both in vitro and in vivo studies were carried out using mesenchymal stem cells (MSCs) and two kinds of gene (luciferase...

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Search results for: Atsuo Ito

Hollow ZnO Nanospheres Enhance Anticancer Immunity by Promoting CD4⁺ and CD8⁺ T Cell Populations In Vivo

Reducing the risk of impaired bone apposition to titanium screws with the use of fibroblast growth factor-2−apatite composite layer coating

Reprint of “Biomimetic apatite coating on yttria-stabilized tetragonal zirconia utilizing femtosecond laser surface processing”

Hollow Structure Improved Anti‐Cancer Immunity of Mesoporous Silica Nanospheres In Vivo

Silica Nanospheres: Hollow Structure Improved Anti‐Cancer Immunity of Mesoporous Silica Nanospheres In Vivo (Small 26/2016)

Biomimetic apatite coating on yttria-stabilized tetragonal zirconia utilizing femtosecond laser surface processing

Controlled superficial assembly of DNA–amorphous calcium phosphate nanocomposite spheres for surface-mediated gene delivery

Rod-shaped and substituted hydroxyapatite nanoparticles stimulating type 1 and 2 cytokine secretion

Stimulation of In Vivo Antitumor Immunity with Hollow Mesoporous Silica Nanospheres

Stimulation of In Vivo Antitumor Immunity with Hollow Mesoporous Silica Nanospheres

Effects of gatifloxaine content in gatifloxacine-loaded PLGA and β-tricalcium phosphate composites on efficacy in treating osteomyelitis

Interlaboratory studies on in vitro test methods for estimating in vivo resorption of calcium phosphate ceramics

Laser-assisted biomimetic process for surface functionalization of titanium metal

Improved gene transfer efficiency of a DNA-lipid-apatite composite layer by controlling the layer molecular composition

Pore size‐dependent immunogenic activity of mesoporous silica‐based adjuvants in cancer immunotherapy

Particle-size-dependent toxicity and immunogenic activity of mesoporous silica-based adjuvants for tumor immunotherapy

Therapeutic effect of zinc‐containing calcium phosphate suspension injection in thermal burn‐rats

Angiogenesis therapy for brain infarction using a slow-releasing drug delivery system for fibroblast growth factor 2

Correlation between cell attachment areas after 2h of culture and osteogenic differentiation activity of rat mesenchymal stem cells on hydroxyapatite substrates with various surface properties

DNA-lipid-apatite composite layers enhance gene expression of mesenchymal stem cells

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