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Recent progress in genome-based breeding has created various fish strains carrying desirable genetic traits; however, methods for the long-term preservation of their genetic resources have not yet been developed, mainly due to the lack of cryopreservation techniques for fish eggs and embryos. Recently, we established an alternative cryopreservation technique for fish spermatogonia using a slow-freezing...
Germ cells prior to sex differentiation are called primordial germ cells (PGCs). They are the progenitor cells of the germ cell lineage and possess the ability to differentiate into either spermatogonia or oogonia. In all animals examined to date, PGCs are set aside from somatic cell lineages during early embryonic development. Recent studies of fish germ cells have revealed that (1) germ plasm-like...
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