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NAnoscopic Ensembles
Diffusion Analysis of NAnoscopic Ensembles (DANAE) offers an assumption‐ and tracking‐free approach to determine the diffusivities of single molecules/particles despite high densities, fast diffusion or photobleaching/blinking, which is possible through a novel treatment of the background function. More details can be found in article number 2206722 by Ulrike Alexiev and co‐workers...
The rapid development of microscopic techniques over the past decades enables the establishment of single molecule fluorescence imaging as a powerful tool in biological and biomedical sciences. Single molecule fluorescence imaging allows to study the chemical, physicochemical, and biological properties of target molecules or particles by tracking their molecular position in the biological environment...
Nanoparticles hold a great promise in biomedical science. However, due to their unique physical and chemical properties they can lead to overproduction of intracellular reactive oxygen species (ROS). As an important mechanism of nanotoxicity, there is a great need for sensitive and high‐throughput adaptable single‐cell ROS detection methods. Here, fluorescence lifetime imaging microscopy (FLIM) is...
In article number 1800310, Nan Ma, Ulrike Alexiev, and co‐workers develop a sensitive and reproducible fluorescence lifetime based imaging assay (FLIM‐ROX) for reactive oxygen species (ROS) detection that allows the investigation of low cellular stress levels as a consequence of ROS production. Using FLIM‐ROX, the nanotoxicity of subcytotoxic amounts of nanoparticles is demonstrated by linking cellular...
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