Search results for: Kanagalaghatta R Rajashankar

Items from 1 to 20 out of 25 results

article

PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease

Hui Yang, Pu Gao, Kanagalaghatta R. Rajashankar, Dinshaw J. Patel

Cell > 2016 > 167 > 7 > 1814-1828.e12

C2c1 is a newly identified guide RNA-mediated type V-B CRISPR-Cas endonuclease that site-specifically targets and cleaves both strands of target DNA. We have determined crystal structures of Alicyclobacillus acidoterrestris C2c1 (AacC2c1) bound to sgRNA as a binary complex and to target DNAs as ternary complexes, thereby capturing catalytically competent conformations of AacC2c1 with both target and...

article

Structures of NS5 Methyltransferase from Zika Virus

Javier Coloma, Rinku Jain, Kanagalaghatta R. Rajashankar, Adolfo García-Sastre, more

Cell Reports > 2016 > 16 > 12 > 3097-3102

The Zika virus (ZIKV) poses a major public health emergency. To aid in the development of antivirals, we present two high-resolution crystal structures of the ZIKV NS5 methyltransferase: one bound to S-adenosylmethionine (SAM) and the other bound to SAM and 7-methyl guanosine diphosphate (7-MeGpp). We identify features of ZIKV NS5 methyltransferase that lend to structure-based antiviral drug discovery...

article

Structural Basis for Phosphatidylinositol-Phosphate Biosynthesis

Meagan L. Belcher Dufrisne, Oliver B. Clarke, David Tomasek, Carla D. Jorge, more

Biophysical Journal > 2016 > 110 > 3 Supplement 1 > 61a

article

ArnT: Structure and Mechanism of the Aminoarabinose Transferase Responsible for Resistance to Polymyxin-Class Antibiotics

Vasileios I. Petrou, Carmen M. Herrera, Kathryn M. Schultz, Oliver B. Clarke, more

Biophysical Journal > 2016 > 110 > 3 Supplement 1 > 38a

article

Crystal Structure of Group II Intron Domain 1 Reveals a Template for RNA Assembly

Chen Zhao, Marco Marcia, Kanagalaghatta R. Rajashankar, Anna Marie Pyle

Biophysical Journal > 2016 > 110 > 3 Supplement 1 > 410a

article

Crystal structure of the Mycobacterium tuberculosis transcriptional regulator Rv0302

Tsung‐Han Chou, Jared A. Delmar, Catherine C. Wright, Nitin Kumar, more

Protein Science > 24 > 12 > 1942 - 1955

Mycobacterium tuberculosis is a pathogenic bacterial species, which is neither Gram positive nor Gram negative. It has a unique cell wall, making it difficult to kill and conferring resistance to antibiotics that disrupt cell wall biosynthesis. Thus, the mycobacterial cell wall is critical to the virulence of these pathogens. Recent work shows that the mycobacterial membrane protein large (MmpL) family...

article

Global RNA Fold and Molecular Recognition for a pfl Riboswitch Bound to ZMP, a Master Regulator of One-Carbon Metabolism

Aiming Ren, Kanagalaghatta R. Rajashankar, Dinshaw J. Patel

Structure > 2015 > 23 > 8 > 1375-1381

ZTP, the pyrophosphorylated analog of ZMP (5-amino-4-imidazole carboxamide ribose-5′-monophosphate), was identified as an alarmone that senses 10-formyl-tetrahydroflate deficiency in bacteria. Recently, a pfl riboswitch was identified that selectively binds ZMP and regulates genes associated with purine biosynthesis and one-carbon metabolism. We report on the structure of the ZMP-bound Thermosinus...

article

Structural Basis for Molecular Discrimination by a 3′,3′-cGAMP Sensing Riboswitch

Aiming Ren, Xin C. Wang, Colleen A. Kellenberger, Kanagalaghatta R. Rajashankar, more

Cell Reports > 2015 > 11 > 4 > 671

article

Structure and Function of Neisseria gonorrhoeae MtrF Illuminates a Class of Antimetabolite Efflux Pumps

Chih-Chia Su, Jani Reddy Bolla, Nitin Kumar, Abhijith Radhakrishnan, more

Cell Reports > 2015 > 11 > 1 > 61-70

Neisseria gonorrhoeae is an obligate human pathogen and the causative agent of the sexually transmitted disease gonorrhea. The control of this disease has been compromised by the increasing proportion of infections due to antibiotic-resistant strains, which are growing at an alarming rate. N. gonorrhoeae MtrF is an integral membrane protein that belongs to the AbgT family of transporters for which...

article

Structural Basis for Molecular Discrimination by a 3′,3′-cGAMP Sensing Riboswitch

Aiming Ren, Xin C. Wang, Colleen A. Kellenberger, Kanagalaghatta R. Rajashankar, more

Cell Reports > 2015 > 11 > 1 > 1-12

Cyclic dinucleotides are second messengers that target the adaptor STING and stimulate the innate immune response in mammals. Besides protein receptors, there are bacterial riboswitches that selectively recognize cyclic dinucleotides. We recently discovered a natural riboswitch that targets 3′,3′-cGAMP, which is distinguished from the endogenous mammalian signal 2′,3′-cGAMP by its backbone connectivity...

article

Crystal Structure of the Bacterial Aminoarabinose Transferase ArnT

Vasileios I. Petrou, Oliver B. Clarke, Kathryn M. Schultz, David Tomasek, more

Biophysical Journal > 2015 > 108 > 2 Supplement 1 > 253a

article

Crystal Structures of Lgr4 and Its Complex with R-Spondin1

Kai Xu, Yan Xu, Kanagalaghatta R. Rajashankar, Dorothea Robev, more

Structure > 2013 > 21 > 9 > 1683-1689

The leucine-rich repeat-containing G-protein-coupled receptors (Lgrs) are a large membrane protein family mediating signaling events during development and in the adult organism. Type 2 Lgrs, including Lgr4, Lgr5, and Lgr6, play crucial roles in embryonic development and in several cancers. They also regulate adult stem cell maintenance via direct association with proteins in the Wnt signaling pathways,...

article

Charged Amino Acids (R83, E567, D617, E625, R669, and K678) of CusA Are Required for Metal Ion Transport in the Cus Efflux System

Chih-Chia Su, Feng Long, Hsiang-Ting Lei, Jani Reddy Bolla, more

Journal of Molecular Biology > 2012 > 422 > 3 > 429-441

Gram-negative bacteria expel various toxic chemicals via tripartite efflux pumps belonging to the resistance–nodulation–cell division superfamily. These pumps span both the inner and outer membranes of the cell. The three components of these tripartite systems are an inner‐membrane, substrate-binding transporter (or pump); a periplasmic membrane fusion protein (or adaptor); and an outer‐membrane-anchored...

article

Purification, crystallization and preliminary X‐ray diffraction analysis of the IL‐20–IL‐20R1–IL‐20R2 complex

Naomi J. Logsdon, Christopher E. Allen, Kanagalaghatta R. Rajashankar, Mark R. Walter

Acta Crystallographica Section F > 68 > 1 > 89 - 92

Interleukin‐20 (IL‐20) is an IL‐10‐family cytokine that regulates innate and adaptive immunity in skin and other tissues. In addition to protecting the host from various external pathogens, dysregulated IL‐20 signaling has been shown to contribute to the pathogenesis of human psoriasis. IL‐20 signals through two cell‐surface receptor heterodimers, IL‐20R1–IL‐20R2 and IL‐22R1–IL‐20R2. In this report,...

article

Structure of Prokaryotic Polyamine Deacetylase Reveals Evolutionary Functional Relationships with Eukaryotic Histone Deacetylases,

Patrick M. Lombardi, Heather D. Angell, Douglas A. Whittington, Erin F. Flynn, more

Biochemistry > 2011 > 50 > 11 > 1808 - 1817

article

HMP Binding Protein ThiY and HMP-P Synthase THI5 Are Structural Homologues,

Shridhar Bale, Kanagalaghatta R. Rajashankar, Kay Perry, Tadhg P. Begley, more

Biochemistry > 2010 > 49 > 41 > 8929 - 8936

article

Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA Capping Apparatus

Meigang Gu, Kanagalaghatta R. Rajashankar, Christopher D. Lima

Structure > 2010 > 18 > 2 > 216-227

The 5′ guanine-N7 cap is the first cotranscriptional modification of messenger RNA. In Saccharomyces cerevisiae, the first two steps in capping are catalyzed by the RNA triphosphatase Cet1 and RNA guanylyltransferase Ceg1, which form a complex that is directly recruited to phosphorylated RNA polymerase II (RNAP IIo), primarily via contacts between RNAP IIo and Ceg1. A 3.0 Å crystal structure of Cet1-Ceg1...

article

Crystal Structure of the Membrane Fusion Protein CusB from Escherichia coli

Chih-Chia Su, Feng Yang, Feng Long, Deepak Reyon, more

Journal of Molecular Biology > 2009 > 393 > 2 > 342-355

Gram-negative bacteria, such as Escherichia coli, frequently utilize tripartite efflux complexes belonging to the resistance–nodulation–division family to expel diverse toxic compounds from the cell. These systems contain a periplasmic membrane fusion protein (MFP) that is critical for substrate transport. We here present the x-ray structures of the CusB MFP from the copper/silver efflux system of...

article

How to Switch Off a Histidine Kinase: Crystal Structure of Geobacillus stearothermophilus KinB with the inhibitor Sda

Matthew J. Bick, Valerie Lamour, Kanagalaghatta R. Rajashankar, Yuliya Gordiyenko, more

Journal of Molecular Biology > 2009 > 386 > 1 > 163-177

Entry to sporulation in bacilli is governed by a histidine kinase phosphorelay, a variation of the predominant signal transduction mechanism in prokaryotes. Sda directly inhibits sporulation histidine kinases in response to DNA damage and replication defects. We determined a 2.0-Å-resolution X-ray crystal structure of the intact cytoplasmic catalytic core [comprising the dimerization and histidine...

article

Structural Similarities between Thiamin-Binding Protein and Thiaminase-I Suggest a Common Ancestor ^† ^, ^‡

Erika V. Soriano, Kanagalaghatta R. Rajashankar, Jeremiah W. Hanes, Shridhar Bale, more

Biochemistry > 2008 > 47 > 5 > 1346 - 1357

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Keywords

SNAKE VENOM (2)
X-RAY CRYSTALLOGRAPHY (2)
PHOSPHOLIPASE A 2 (1)
ADAPTOR–TRANSPORTER COMPLEX (1)
BINARY COMPLEX WITH SGRNA (1)
C2C1 (1)
DIFFERENCE SPECTROSCOPY (1)
EFFLUX PUMP (1)
ESCHERICHIA COLI (1)
GENOME EDITING TOOL (1)
HEAVY-METAL RESISTANCE (1)
HEAVY‐METAL RESISTANCE (1)
HISTIDINE KINASE (1)
IL‐20R1 (1)
IL‐20R2 (1)
INTERLEUKIN‐20 (1)
MEMBRANE FUSION PROTEIN (1)
MEMBRANE PROTEIN (1)
MYCOBACTERIAL MEMBRANE PROTEIN (1)
MYCOBACTERIAL MEMBRANE PROTEIN LARGE (1)
NEUROTOXIN (1)
PHOSPHOLIPASE A2 (1)
RESISTANCE–NODULATION–CELL DIVISION (1)
RNA (1)
RUVC CATALYTIC POCKET (1)
RV0302 (1)
SDA (1)
SEQUENCE-SPECIFIC PAM RECOGNITION (1)
SMALL (1)
STRUCTURE (1)
TERNARY COMPLEX WITH ADDED DNA (1)
TETR‐FAMILY REGULATOR (1)
TITRATION (1)
TOXICITY (1)
TRANSPORT (1)
TYPE V CRISPR-CAS ENDONUCLEASE (1)
TYROSINE IONIZATION (1)
X-RAY MODEL (1)
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Data set

Elsevier (20)
ACS (3)
Wiley (2)

Journal

Biophysical Journal (4)
Cell Reports (4)
Structure (4)
Biochemistry (3)
Journal of Molecular Biology (3)
Neuron (2)
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2)
Acta Crystallographica Section F (1)
Cell (1)
Protein Science (1)
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INFONA - science communication portal

Search results for: Kanagalaghatta R Rajashankar

PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease

Structures of NS5 Methyltransferase from Zika Virus

Structural Basis for Phosphatidylinositol-Phosphate Biosynthesis

ArnT: Structure and Mechanism of the Aminoarabinose Transferase Responsible for Resistance to Polymyxin-Class Antibiotics

Crystal Structure of Group II Intron Domain 1 Reveals a Template for RNA Assembly

Crystal structure of the Mycobacterium tuberculosis transcriptional regulator Rv0302

Global RNA Fold and Molecular Recognition for a pfl Riboswitch Bound to ZMP, a Master Regulator of One-Carbon Metabolism

Structural Basis for Molecular Discrimination by a 3′,3′-cGAMP Sensing Riboswitch

Structure and Function of Neisseria gonorrhoeae MtrF Illuminates a Class of Antimetabolite Efflux Pumps

Structural Basis for Molecular Discrimination by a 3′,3′-cGAMP Sensing Riboswitch

Crystal Structure of the Bacterial Aminoarabinose Transferase ArnT

Crystal Structures of Lgr4 and Its Complex with R-Spondin1

Charged Amino Acids (R83, E567, D617, E625, R669, and K678) of CusA Are Required for Metal Ion Transport in the Cus Efflux System

Purification, crystallization and preliminary X‐ray diffraction analysis of the IL‐20–IL‐20R1–IL‐20R2 complex

Structure of Prokaryotic Polyamine Deacetylase Reveals Evolutionary Functional Relationships with Eukaryotic Histone Deacetylases,

HMP Binding Protein ThiY and HMP-P Synthase THI5 Are Structural Homologues,

Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA Capping Apparatus

Crystal Structure of the Membrane Fusion Protein CusB from Escherichia coli

How to Switch Off a Histidine Kinase: Crystal Structure of Geobacillus stearothermophilus KinB with the inhibitor Sda

Structural Similarities between Thiamin-Binding Protein and Thiaminase-I Suggest a Common Ancestor ^† ^, ^‡

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