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Attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS) has recently been proven to be a powerful tool for bioanalysis. It enables in situ and in real-time observation of dynamic processes occurring on specific interface, revealing rich structural and functional information of biomolecules at sub monolayer level. The aim of this general review was to give an overview...
The anti‐enzymolysis capability of surface attached DNA, morpholino (MO) single strands, and DNA‐DNA, MO‐DNA double strands are studied using cyclic voltammetry. Results show that MO, the neutral analog of DNA, bears strong anti‐enzymolysis capability against DNase I and can protect its complementary DNA from being decomposed. Only 15 % of the DNA in the MO‐DNA double strands is decomposed within...
A novel assay for the voltammetric detection of 18-bases DNA sequences relating to Chronic Myelogenous Leukemia (CML, Type b3a2) using methylene blue (MB) as the hybridization indicator was reported. DNA was covalently attached onto a glassy carbon electrode (GCE) through amines of the DNA bases using N-hydroxysulfosuccinimide (NHS) and N-(3-dimethylamion)propyl-N′-ethyl carbodiimidehydrochloride...
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