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(Coβ-5'-Deoxyadenosin-5'-yl)-(p-cresyl)cobamide (Ado-PCC), a base-off analogue of coenzyme B 12 (Ado-Cbl), was used to elucidate the coenzyme B 12 binding mode of glutamate mutase, 2-methyleneglutarate mutase and ethanolamine ammonia-lyase (EAL). Ado-PCC functions as excellent coenzyme for the carbon skeleton rearrangements with apparent K m values of 200 and 10 nM...
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