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Objectives To develop a new vector for constitutive expression in Pichia pastoris based on the endogenous glycolytic PGK1 promoter. Results P. pastoris plasmids bearing at least 415 bp of PGK1 promoter sequences can be used to drive plasmid integration by addition at this locus without affecting cell growth. Based on this result, a new P. pastoris integrative vector, pPICK2, was constructed...
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