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For molecular biological detection of Mycobacterium tuberculosis, PCR methods with primers targeting different regions specific for mycobacterium tuberculosis complex are used worldwide. However, some uncertainties exist concerning the specificity of certain target regions and the sensitivity. To identify the methods which are best suited for diagnostics, 9 single-round and 2 multiple PCR systems...
In vivo, cardiomyocytes are stimulated by electricity continuously. So electrical stimulation is an indispensable factor for cardiomyocytes cultured in vitro. In this study, the experimental group neonatal cardiomyocytes received electrical stimulation at different intensities and different frequencies by a electrical stimulation device in vitro. After stimulation, The morphology and beating of cardiomyocytes...
Mechanical stimulations are critical factors in regulation activities of cardiomyocytes in vivo and in vitro. Cyclic mechanical stretch is an indispensable factor for cardiomyocytes cultured in vitro and construction of engineered cardiac tissue. Optimized parameter of cyclic stretch is key factor for cardiomyocytes culture in vitro. In this study, neonatal cardiomyocytes were subjected to cyclic...
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