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A proximity hybridization-regulated electrogenerated chemiluminescence (PLA-ECL) bioassay was developed for the detection of α-fetoprotein (AFP) on basis of the sensitization of gold nanoparticles (AuNPs) and target-induced quenching mechanism. Ru(bpy)32+ was used as ECL signal while ferrocene (Fc) was used as ECL quencher. Ru(bpy)32+ was electrostatically adsorbed into the AuNPs/Nafion film prepared...
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