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AbstractDihydroxyacetone synthase (EC 2.2.1.3), which is a key enzyme of the C1compound-assimilating pathway in yeasts, catalyzes transketolation between formaldehyde and hydroxypyruvate, leading to the formation of dihydroxyacetone and CO2. When [13C]formaldehyde was used as a substrate with dihydroxyacetone synthase from Candida boidinii 2201, 13C was confirmed to be incorporated to the C-1 and...
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