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Aims: For the rapid detection of Pseudomonas aeruginosa from chlorinated water and aerosols, gyrB gene‐based real‐time PCR assay was developed and investigated.
Methods and Results: Two novel primer sets (pa722F/746MGB/899R and pa722F/746MGB/788R) were designed using the most updated 611 Pseudomonas and 748 other bacterial gyrB genes for achieving high specificity. Their specificity showed 100%...
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