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A full-length cDNA copy of the RNA genome of bacteriophage MS2 was assembled by the in-frame ligation of the central portion of the genome into a plasmid containing the 5′ and 3′ ends. Upon transformation of the ligation reaction into Escherichia coli, infectious phage particles were released into the medium. The plaquing ability of the phage produced from the cDNA construct was assessed against various...
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