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One hundred and seventy-six samples collected from internal organs of sick birds and 40 samples taken from healthy birds were used for virus isolation. The isolation was carried out on SPF chicken embryos by inoculating them into the yolk sac with the material prepared by generally accepted methods. Three passages were performed. The presence of viral antigen was detected by the agar gel precipitation...
This work aims to identify 6 virus field isolates, serologically determined as reoviruses. The type of cytopathic effect in cell cultures, physico-chemical properties and antigenic relationship were examined. The strains examined multiplied in CEF and CEK cultures, produced cytopathic effects and plaques, contained RNA in their genome, had no lipid envelope, were stable in an acid environment and...
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