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A fluorescent probe (RB–S) containing the N-hydroxysuccinimidyl ester in 2 position was prepared from rhodamine B (RB) by one-step condensation reaction. The reactivity of the fluorescent probe with glycine was studied and the products were identified by LC–MS analysis. The excessive equivalent of the RB–S was hydrolyzed to the corresponding carboxylic acids (RB). UV/vis and fluorescence spectra of...
N-Hydroxysuccinimidyl benzoate was prepared as a model compound of fluorescent probes. Its crystal structure was determined. The interlayer space between neighbored aromatic rings is inserted by the carbonyl group of succinimidyl moiety and the stacking distance between aromatic rings is as far as 5.9085Å. Labeling activity of carbonyl groups in N-hydroxysuccinimidyl benzoate was studied by AM1 semiempirical...
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