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A virial theory was used to relate molecular interactions (in terms of second virial coefficients, B′) and molecular size ratios to liquid–liquid phase separation. Application of the virial theory to binary hard sphere mixtures (additive and non-additive) confirmed the applicability of this simple approach towards predicting phase behavior based on two-particle interactions.Experimentally, second...
The interaction of proteins (β-lactoglobulin, Bovine Serum Albumin (BSA), gelatins and whey protein isolate (WPI)) in solution was quantified by measuring their second virial coefficient using membrane osmometry. At neutral pH below 20–40 mM ionic strength, electrostatic repulsion dominated the interaction. At higher ionic strength, BSA, WPI and whey protein aggregates (WPA) were well approximated...
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