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Here, we present an update of the CHARMM27 all‐atom additive force field for nucleic acids that improves the treatment of RNA molecules. The original CHARMM27 force field parameters exhibit enhanced Watson‐Crick base pair opening which is not consistent with experiment, whereas analysis of molecular dynamics (MD) simulations show the 2′‐hydroxyl moiety to almost exclusively sample the O3′ orientation...
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