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Autographa californica multiple nucleopolyhedrovirus (AcMNPV) orf53 (ac53) is a highly conserved gene existing in all sequenced Lepidoptera and Hymenoptera baculoviruses, but its function remains unknown. To investigate its role in the baculovirus life cycle, an ac53 deletion virus (vAc ac53KO-PH-GFP ) was generated through homologous recombination in Escherichia coli. Fluorescence and light...
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) p48 (ac103) is a highly conserved baculovirus gene of unknown function. In the present study, we generated a knockout of the p48 gene in an AcMNPV bacmid and investigated the role of P48 in baculovirus life cycle. The p48-null Bacmid vAc P48-KO-PH-GFP was unable to propagate in cell culture, while a ‘repair’ Bacmid vAc P48-REP-PH-GFP...
ChaB, a putative regulator of ChaA in Escherichia coli (E. coli), and its homologues constitute a multigene family known to occur among bacteria, archaeabacteria and baculoviruses. The baculovirus Spodoptera exigua multicapsid nucleopolyhedrovirus contains two homologues of E. coli ChaB, open reading frames (ORFs) 100 (Se100) and 101 (Se101). RT-PCR and 5′ RACE analyses indicated that transcription...
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