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A surface plasmon resonance (SPR) protocol is described for the direct kinetic analysis of small antigenic peptides interacting with immobilized monoclonal antibodies (mAb). High peptide concentrations (up to 2.5 μM) and medium mAb surface densities (about 1.5 ng/mm 2 ) are needed to ensure measurable binding levels, and fast buffer flow rates (60 μl/min) are required to minimize diffusion-controlled...
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