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We describe an experimental model for human bone formation in unloaded conditions. Bone formation has been assessed by implanting in vivo human bone marrow stromal cells (BMSC) on porous hydroxyapatite (HA) bioceramics subcutaneously in nude mice. In this system, human bone formation and remodeling occurs and can be studied in unloaded conditions, i.e., with no influence of muscle tension. Using this...
Factors that regulate growth and development of mesenchymal precursor cells towards chondrogenesis are not well identified. In the present study, we have developped a defined serum-free culture system which allows proliferation of chicken embryo tibia prechondrogenic cells (stage H.H. 28-30) and their subsequent maturation towards hypertrophic chondrocytes. Cell proliferation was obtained in adherent...
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