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The immunocytokine scFvMEL/TNF is a fusion protein composed of tumor necrosis factor (TNF-α) and a single-chain Fv antibody scFvMEL targeting the melanoma-associated gp240 antigen. The fusion protein containing thioredoxin and a hexa-histidine tag was expressed in two Escherichia coli host cells, AD494 (DE3) pLysS and T7 Express Iq. The cell growth and expression level of target protein, His-tagged...
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