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Key components of vesicular neurotransmitter release, such as Ca 2+ influx and membrane recycling, are affected by cytosolic pH. We measured the pH-sensitive fluorescence of Yellow Fluorescent Protein transgenically expressed in mouse motor nerve terminals, and report that Ca 2+ influx elicited by action potential trains (12.5–100 Hz) evokes a biphasic pH change: a brief acidification...
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