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A labeled variant of MSH(4), a tetrapeptide that binds to the human melanocortin 4 receptor (hMC4R) with low μM affinity, was prepared by solid-phase synthesis methods, purified, and characterized. The labeled ligand, Eu-DTPA-PEGO-His-dPhe-Arg-Trp-NH 2 , exhibited a K d for hMC4R of 9.1±1.4μM, approximately 10-fold lower affinity than the parental ligand. The labeled MSH(4) derivative...
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