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Previously, we used a simple, sensitive agar plate method to screen lipase activity from 1229 selected cultures, including 508 bacteria, 479 yeasts, 230 actinomycetes and 12 fungi, that covered many genera and species. About 25% of the cultures tested were lipase-positive. We also expanded our screening method to focus specifically on the pH dependence and thermostability of these lipase activities...
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