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In the presence of neostigmine (0.1 μM), S-isopetasin competitively antagonized cumulative acetylcholine-induced contractions in guinea pig trachealis, because the slope [1.18±0.15 (n=6)] of Schild's plot did not significantly differ from unity. The pA2 value of S-isopetasin was calculated to be 4.62±0.05 (n=18). The receptor binding assay for muscarinic receptors of cultured human tracheal smooth...
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