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A recombinant strain Escherichia coli SW0209-52 was immobilized with κ-carrageenan for the production of l-phenylalanine via phenylpyruvic acid (PPA) and l-aspartic acid (l-Asp). To enhance operational stability, the immobilized cells were treated with 0.05% (w/v) glutaraldehyde and the relative activity was 122.8% compared with that without treatment. The conversion yield could stay above 88% in...
Fusarium moniliforme CGMCC 0536 showing excellent stereoselective d-pantonohydrolase activity was immobilized by cross-linking with glutaraldehyde. The stereospecificity of the reaction system were not affected after cross-linking. The feasibility of continuous asymmetric hydrolysis of dl-pantolactone coupled to the separation of biocatalyst on-line in a membrane bioreactor was investigated. The influence...
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