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A novel metabolomics approach for NMR‐based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of 13C‐satellite peaks using 1D‐1H‐NMR spectra. In comparison with 13C‐NMR, TOCSY and HSQC, PEPA improves...
Wie chemische Spezies den komplexen Satz metabolischer Reaktionen in einer Zelle durchlaufen, ist eine wichtige Frage, um die Regulation des Zellstoffwechsels bei Gesundheits‐ und Krankheitszuständen zu verstehen. M. Vinaixa, O. Yanes et al. beschreiben in der Zuschrift auf S. 3585 ff. eine neuartige 1D‐1H‐NMR‐Hochdurchsatzmethode für die Detektion stabiler Isotope in der Metabolomik. Der Ansatz erweitert...
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