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Background: To determine glycoserum, the nitroblue tetrazolium (NBT) assay is quick, economical, convenient and easily automated. This method, however, is vulnerable to interference by thiol group, which should not be ignored during the analysis. Methods: Thiol group in glycoserum was blocked with iodoacetamide (IAM) before NBT was added. The reaction was carried out in a thermal bath and terminated...
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