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Efferents, originating in the superior olivary complex, preferentially synapse with cochlear outer hair cells (OHCs), with acetylcholine (ACh) as their primary neurotransmitter. The OHC ACh receptors (AChRs), which have unusual pharmacology, have been cloned and identified as a new subunit (α9) of the nicotinic AChR family. The expression of α9 AChRs is first detected before birth and peaks between...
In order to identify hair cell specific genes, it is essential to obtain isolated hair cells in quantity. While whole-cell recordings have been made from isolated inner hair cells (IHCs) from guinea pigs, detailed methods for obtaining a fairly large amount of isolated inner hair cells have not been published. Here we describe a protocol that can yield a fairly large amount of isolated gerbil IHCs...
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