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Light is indispensable in many biomedical reactions, but there are few nanomaterials capable of light‐activated enzyme‐like activities. In this work, carbon dots (C‐dots) were prepared through one‐step pulsed laser ablation (PLA) treatment of polymers in aqueous solution. The choice of water‐soluble polymers as starting materials could extend the types of carbon sources for PLA reactions. The obtained...